Table 3.

Parameters obtained from global fitting of PLP inhibition data obtained with ePNPO R23L/R24L, R23L/R215L, and R23L/R24L/R215L variants

Parameters	aWT	R23L/R24L	R23L/R215L	R23L/R24L/R215L
VMAX (μM s−1)	0.135 ± 0.005	0.074 ± 0.001	0.016 ± 0.001	0.037 ± 0.001
KM (μM)	0.9 ± 0.55	0.80 ± 0.08	5.40 ± 0.22	3.19 ± 0.09
β	-	0.42 ± 0.05	-	-
KI (μM)	0.59 ± 0.7	0.82 ± 0.02	13.28 ± 2.45	-
αKI (μM)	7.13 ± 0.52	5.39 ± 1.14	-	-
KX (μM)	-	6.58 ± 0.62	-	1.78 ± 0.06
γKX (μM)	-	2.05 ± 0.06	0.73 ± 0.14	-

Missing parameters do not occur in the models used to fit experimental data.

^aDetermined according to Figure 2A by Barile et al. (5).