Skip to main content
. 2021 Jun 12;14(9):101148. doi: 10.1016/j.tranon.2021.101148

Fig. 1.

Fig 1

The effect of CDK5 disruption or overexpression on PD-L1 expression in LUAD cells. A. The expression levels of CDK5 and PD-L1 protein detected by western blot in A549 and NCI-H1975 cells after CDK5 knockdown. B. The expression levels of CDK5 mRNA detected by qPCR in A549 and NCI-H1975 cells after CDK5 knockdown. *** p < 0.005, **** p < 0.0005. C. qRT-PCR was used to evaluate the PD-L1 mRNA expression in CDK5 deficient cells. NS: not significant. D. The expression levels of CDK5 protein detected by western blot in LTEP-a-2 and A549 cells after CDK5 overexpression. E. The expression levels of CDK5 mRNA detected by qPCR in LTEP-a-2 and A549 cells after CDK5 overexpression. ** p < 0.01, *** p < 0.005. F. The expression levels of PD-L1 mRNA detected by qPCR in LTEP-a-2 and A549 cells after CDK5 overexpression. NS: not significant. G. Western blot analysis of PD-L1 protein levels in dinaciclib treated cells. H. Western blot measurement of PD-L1 after cells treated with different concentration of dinaciclib in NCI-H1975. I. Quantification of PD-L1 mRNA expression by using qRT-PCR in dinaciclib treated cells. NS: not significant. *p < 0.05.