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. 2021 Jun 7;12:693974. doi: 10.3389/fimmu.2021.693974

Figure 5.

Figure 5

rCLEC-2-Fc increases peritoneal macrophage migration to mesenteric lymph nodes during endotoxemia. WT mice were intraperitoneally injected with LPS (10mg/kg) for 18h followed by rCLEC-2-Fc or IgG isotype control (100µg/mouse) for an additional 4h (n=4). (A–E) Mesenteric lymph node (MLN) cells were collected and immune cell population detected by flow cytometry. (A) Percentage of myeloid cells (CD45+CD11b+) and (B) macrophages (CD45+CD11b+F4/80+). (C) CLEC-2-positive macrophages and (D) the MFI of CLEC-2 expression on macrophages in MLN. (E) Correlation between the percentage of MLN macrophages and the MFI of CLEC-2 expression on macrophages by Simple Linear Regression. (F) Immunofluorescent staining of macrophages (F4/80; purple) and platelets (CD41; green) in frozen MLN sections. (G) Immunofluorescent staining of macrophages (F4/80; blue), CLEC-2 (green) and podoplanin (orange) in frozen MLN sections. The statistical significance was analyzed using a Kruskal-Wallis multiple comparisons test. *p < 0.05.