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. 2021 Jun 7;12:652923. doi: 10.3389/fimmu.2021.652923

Figure 2.

Figure 2

Effect of porcine Ligilactobacillus salivarius strains on the expression of immune factors in porcine intestinal epithelial (PIE) cells. PIE cells were stimulated with L. salivarius FFIG35, FFIG56 or FFIG58 isolated form the porcine gastrointestinal tract and the expression of Toll-like receptor (TLR)-2, TLR3, TLR4, nucleotide-binding oligomerization domain-containing protein (NOD)-1, NOD2, protein kinase R (PKR), retinoic acid-inducible gene I (RIG-I), interferon (IFN)-β, IFN-λ3, IFN-induced GTP-binding protein Mx1 (Mx1), ribonuclease L (RNAseL), 2’-5’-oligoadenylate synthetase 1 (OAS1), tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-8, IL-12, IL-18 and monocyte chemoattractant protein 1 (MCP-1) were determined by RT-qPCR at the indicated time points. After normalization of genes with β-actin, the relative expression compared to the expression of each gene in the control was calculated. The results represent data from three independent experiments at each time point. Values are means ± SD. Asterisks indicate significant differences when compared to hour 0 (*P < 0.05, **P < 0.01).