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. 2021 Jun 7;12:652923. doi: 10.3389/fimmu.2021.652923

Figure 4.

Figure 4

Effect of porcine Ligilactobacillus salivarius strains on the expression of negative regulators of the Toll-like receptor (TLR) signaling pathway in porcine intestinal epithelial (PIE) cells in response to TLR3 activation. PIE cells were stimulated with L. salivarius FFIG35, FFIG56 or FFIG58 isolated form the porcine gastrointestinal tract and then challenged with poly(I:C) to activate TLR3. The expression of zinc finger protein A20 (A20), B-cell lymphoma-3 (Bcl-3), Toll interacting protein (Tollip) and interleukin-1 receptor-associated kinase M (IRAK-M) were determined by RT-qPCR after 3, 6 or 12 hours of TLR3 activation. PIE cells with no challenge (basal control) or stimulated only with poly(I:C) (poly(I:C) control) were used for comparisons. After normalization of genes with β-actin, the relative expression compared to the expression of each gene in the poly(I:C) control was calculated. The results represent data from three independent experiments. Values are means ± SD. Asterisks indicate significant differences when compared to the poly(I:C) control group (*P < 0.05, **P < 0.01).