Skip to main content
. 2021 Jun 7;12:652923. doi: 10.3389/fimmu.2021.652923

Figure 8.

Figure 8

Effect of porcine Ligilactobacillus salivarius strains on the expression of immune factors in porcine intestinal epithelial (PIE) cells in response to enterotoxigenic Escherichia coli (ETEC) and rotavirus infection. PIE cells were stimulated with L. salivarius FFIG35 or FFIG58 isolated form the porcine gastrointestinal tract and then challenged with ETEC F6 and rotavirus. The expression of interferon (IFN)-β, IFN-λ3, protein kinase R (PKR), IFN-induced GTP-binding protein Mx1 (Mx1), ribonuclease L (RNAseL), 2’-5’-oligoadenylate synthetase 1 (OAS1), interleukin (IL)-6, IL-8 and monocyte chemoattractant protein 1 (MCP-1), zinc finger protein A20 (A20), B-cell lymphoma-3 (Bcl-3), Toll interacting protein (Tollip), interleukin-1 receptor-associated kinase M (IRAK-M), mitogen-activated protein kinase phosphatase-1 (MKP-1) and single immunoglobulin interleukin-1 related receptor (SIGIRR) were determined by RT-qPCR after 3, 6 or 12 hours of ETEC and rotavirus infection. PIE cells with no lactobacilli treatment and challenged with ETEC and rotavirus were used for comparisons. After normalization of genes with β-actin, the relative expression compared to the expression of each gene in the ETEC, and rotavirus control was calculated. The results represent data from three independent experiments at each time point. Values are means ± SD. Asterisks indicate significant differences when compared to the ETEC and rotavirus control group (*P < 0.05, **P < 0.01).