FIGURE 3.

JAM-C decreases cytosolic Ca²⁺ concentrations in human RCECs. Cultured human RCECs were treated with VEGF (10 ng/mL) or PDGF-C (125 ng/mL) for 1 h. Cytosolic Ca²⁺ concentrations were detected by flow cytometry. (A) VEGF and PDGF-C increased cytosolic Ca²⁺ concentrations in RCECs. (B) JAM-C overexpression decreased cytosolic Ca²⁺ concentrations, whereas JAM-C knockdown increased Ca²⁺ concentrations in RCECs. VEGF and PDGF-C did not increase calcium influx in RCECs after JAM-C knockdown. *P < 0.05, **P < 0.01, NS, not significant.