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. 2021 Jun 7;9:675486. doi: 10.3389/fcell.2021.675486

FIGURE 7.

FIGURE 7

ISRIB and CPE treatment exerted anti-hypertrophy and anti-apoptosis effects of NP cells in vivo. The mice were treated with the procedure described above. (A) Representative micrographs of slices stained with eIF2α, p-eIF2α, ATF4, Ihh, MMP13, SOD1, and Caspase3. (B) Quantitation of percentage of total positive cells (%) (scale bar = 50 μm). Data are presented as means ± SD (n = 6). *p < 0.05; **p < 0.01; ***p < 0.001; the orange column indicates control mice without lumbar surgery to induce instability, the green column indicates mice injected with NS after lumbar surgery, the red column indicates mice injected with ISRIB after lumbar surgery, the blue column indicates mice injected with CPE after lumbar surgery, and the gray column indicates mice injected with ISRIB and CPE after lumbar surgery. CPE, cyclopamine; NP, nucleus pulposus; eIF2α, eukaryotic initiation factor-2α; p-eIF2α, phosphorylated eukaryotic initiation factor-2α; ATF4, activating transcription factor 4; Ihh, Indian hedgehog; MMP13, matrix metalloproteinase-13; SOD1, superoxide dismutase 1.