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. 2021 Mar 25;38(1):31–36. doi: 10.5511/plantbiotechnology.20.0910a

Figure 1. Two types of artificial seeds. (A) Bead type: Murashige and Skoog (MS) medium containing 1% sodium alginate and a somatic embryo was dropped into MS medium containing 1% CaCl2. The alginate bead with an embryo was formed during incubation in 1% CaCl2. (B) Sponge type: MS medium containing 1% sodium alginate and 4% gelatin was whipped and dropped into MS medium containing 1% CaCl2 and a somatic embryo was placed in the floating whipped medium. The whipped alginate gel with an embryo was formed by immersing the whipped medium in 1% CaCl2. Finally, both seeds were washed with MS medium.

Figure 1. Two types of artificial seeds. (A) Bead type: Murashige and Skoog (MS) medium containing 1% sodium alginate and a somatic embryo was dropped into MS medium containing 1% CaCl2. The alginate bead with an embryo was formed during incubation in 1% CaCl2. (B) Sponge type: MS medium containing 1% sodium alginate and 4% gelatin was whipped and dropped into MS medium containing 1% CaCl2 and a somatic embryo was placed in the floating whipped medium. The whipped alginate gel with an embryo was formed by immersing the whipped medium in 1% CaCl2. Finally, both seeds were washed with MS medium.