Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jun 7;9:684885. doi: 10.3389/fcell.2021.684885

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Gong, Wang, Wang, Hu, Li, Gao and Lin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

CRL5 interacts with p62 and regulates its expression. (A) HepG2, SNU739, and Huh1 cells were treated with 10 μM MG132, 1 μM MLN4924, or 200 nM Baf A1 for 10 h, and the whole-cell lysates (WCL) were generated for immunoblotting (IB) analysis. p27 served as a positive control responding to CRL E3 blocking, and Tubulin served as a loading control. (B) Huh1 cells infected with indicated shRNAs were subjected to IB analysis. Tubulin served as a loading control. (C) IB analyses of WCL from HeLa cells transfected with plasmids encoding HA-p62, shRNAs targeting EloB/C, and GFP (as an internal transfection control). Tubulin served as a loading control. (D,E) HEK293T cells transfected with the indicated plasmids were treated with 10 μM MG132 for 10 h before Co-IP and IB analysis.