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. 2021 Jun 7;9:684885. doi: 10.3389/fcell.2021.684885

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Copyright © 2021 Gong, Wang, Wang, Hu, Li, Gao and Lin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CUL5–ASB6 complex interacts with and regulates short p62 isoform. (A) Schematic representation of the domain composition of p62 and p62S. (B,C) HEK293T cells transfected with the indicated plasmids were treated with 10 μM MG132 for 10 h before harvest for IP and IB analysis. (D) HEK293T cells were co-transfected with plasmids encoding HA-p62S, shRNAs targeting CUL5, and GFP (as an internal transfection control), and were harvested for IB analysis 48 h later. Tubulin served as a loading control. (E) IB analysis of HEK293T cells transfected with HA-p62S plasmid and increasing amount of Myc-CUL5 construct. Tubulin served as a loading control.