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. 2021 Jun 7;9:684885. doi: 10.3389/fcell.2021.684885

FIGURE 5.

FIGURE 5

The degradation of p62 by ASB6 is independent of autophagy. (A) IB analysis of p62 levels in ATG7–/– MEF cells stably expressing ectopic ASB6. Tubulin was used as a loading control. (B) ATG7–/– MEF cells stably expressing ectopic ASB6 were treated with 50 μg/ml CHX and harvested at indicated time for IB analysis. Tubulin served as loading control. (C) ATG7–/– MEF cells stably expressing ectopic ASB6 were counted and seeded into 96-well plates (500 cells per well) to perform CCK8 experiment at the indicated time. Data were shown as mean ± SEM (n = 3). Statistical analyses were performed using Student’s t-test. **P < 0.01, ***P < 0.001. (D) ATG7–/– MEF cells stably overexpressing ASB6 were counted and seeded into six-well plates (500 cells per well). The number of colonies were measured and analyzed after 10 days. Data represent the mean ± SEM. **P < 0.01, by Student’s t-test.