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. Author manuscript; available in PMC: 2022 Jun 1.
Published in final edited form as: Clin Immunol. 2021 May 1;227:108752. doi: 10.1016/j.clim.2021.108752

Figure 4. Correlation and Receiver operating characteristic (ROC) analysis of IgE receptor (FCεRII), iNKT cell receptor (Jα18) and T Cell receptor (TCRδ) to discriminate between patients with EoE compared to GERD.

Figure 4.

A significant statistical correlation between maximum eosinophils number/hpf and FcεRII, Jα18, and δ TCR m RNA expression in human EoE is shown (A-C). p value and r were calculated using Spearman’s correlation test, mRNA vs eosinophils **** p<0.0001. The solid line (no discrimination) indicates values that have no discriminatory value. Note that on the vertical axis, the scale is from no (0%) to complete (100%) sensitivity. The horizontal axis is a reciprocal scale (100% - % specificity). Based on the analysis, the following cut-off points of the individual biomarkers were chosen as an mRNA relative expression normalized with GAPDH of each molecule: A- FcεRII (0.75), B- Jα18 (1.32), and C- δ TCR (3.01). The use of all three biomarkers in individual yielded an excellent positive predictive value for D- FcεRII (100%), E- Jα18 (83%) and F- δ TCR (95%) (D-F). The optimum performance of a diagnostic test is determined either at the highest sum of the sensitivity and specificity or at an acceptable level of sensitivity for the given disease.