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. 2021 Apr 5;42(6):814–825. doi: 10.1093/carcin/bgab027

Figure 3.

Figure 3.

DKK1 expression correlates with 5FU resistance in primary CRC cancers and CRC cell lines. (A) Heatmap of the transcriptional expression of Wnt/β-catenin pathway-specific genes in GSE52735. (B) Scatter plots of DKK1 expression in 14 nonresponder and 23 responder patients treated with 5FU-based chemotherapy. (C) Bar graph showing relative mRNA expression of DKK1 in HCT116 5FUR cells and SW480 5FUR cells compared with their respective parental cells by qPCR analysis. (D) Representative images of DKK1 protein expression in HCT116 5FUR cells and SW480 5FUR cells compared with their respective parental cells by western blot analysis. (E) Heatmap of common differentially expressed genes in both SW480 5FUR and HCT116 5FUR cells (|log2 fold change | > 1, P < 0.05). (F) Representative images of DKK1 protein expression in HCT116 5FUR cells and SW480 5FUR with or without andrographis treatment. Statistical significance: *P < 0.05, ***P < 0.001. Andro, andrographis.