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. 2021 Apr 5;42(6):814–825. doi: 10.1093/carcin/bgab027

Figure 4.

Figure 4.

Silencing DKK1 sensitizes 5FUR cells to 5FU via regulating Akt-phosphorylation. (A) Bar graph showing relative DKK1 mRNA expression of 5FUR cells transfected with negative control siRNA or DKK1 siRNA by qPCR analysis. (B) Representative images of DKK1 protein expression of 5FUR cells transfected with negative control siRNA or DKK1 siRNA by western blot analysis. (C) Results of CCK-8 assay showing the effects of 5FU on 5FUR cells transfected with negative control siRNA or DKK1 siRNA. The complete medium containing 5FU (10 µM) was added at 24 h, and the absorbances at 450 nm were measured after culture for 0, 12, 24, 36, 48, 60 and 72 h. (D) Representative images of Akt and pAkt expression of 5FUR cells transfected with negative control siRNA or DKK1 siRNA. (E) Representative images of DKK1, Akt and pAkt expression of 5FUR cells in each treatment group, and the phosphorylation of Akt was analyzed as quotient of pAkt versus Akt expression in the right panel. Statistical significance: **P < 0.01, ***P < 0.001. Andro, andrographis; pAkt, phosphorylated-Serine473-Akt.