FIGURE 2.

Screening of viral sequences for responsiveness to selected miRNAs in the A549 cell line. SARS-CoV-2 sequences predicted to be targeted by the indicated miRNAs were cloned in the reporter vector psiCheck-2. 48 h after transfection, luciferase activities were recorded; the Renilla luciferase activity (Rl) was normalized to the firefly luciferase activity (Luc), whose gene is also contained in the reporter vector; the values are reported as fold mean value ± s.d. relative to the luciferase activity determined for parental vector psiCheck-2 transfection (V), which was set to 1. p-values at Student’s t-test were *p < 0.05, **p < 0.01, or ***p < 0.001.