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. 2021 Jun 7;12:678994. doi: 10.3389/fgene.2021.678994

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Copyright © 2021 Siniscalchi, Di Palo, Russo and Potenza.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Silencing effects toward viral sequences are modulated by synthetic miRNA mimics. (A) A549 cells were transfected with the luciferase-based reporter constructs containing the viral target sequence of indicated miRNA (WT-miRNA abbreviation name) or the control plasmids with inverted target sequence (I-miRNA abbreviation name), along with 50 nM (+) of the specific miRNA mimic, or unrelated molecule used as negative control, Ctrl-miRNA. The values are reported as fold mean ± s.d. relative to Rl/Luc recorded for transfections of control molecules of each series (specific I construct and Ctrl-miRNA), which were set to 1. (B) A549 were transfected with Spike-expressing plasmid (pSpike) along with Ctrl-miRNA or miR-15b; 48 h after transfection, Spike expression was quantitated by RT-qPCR. Data are the mean ± s.d. of two independent experiments, each with three data sets. *, **, or ***, p-values as indicated in Figure 2.