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Effects of nitrate pretreatment on NRF2 activation in ischemia-induced mice. (A) Relative mRNA level of Nrf2, Keap1, Nqo1, Ho1, TrxR, GSTp1, CAT, GPX1. (B) Western blot analysis of total NRF2, KEAP1, NQO1, HO1 and (C) statistical analyses. (D) Western blot analysis of cytosolic NRF2 and (E) statistical analyses. (F) Western blot analysis of nuclear NRF2 and (G) statistical analyses. Data are expressed as mean ± SEM, *p<0.05, **p<0.01, ***p<0.001, and NS denotes no significance.
