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Effect of Roflumilast on adipogenesis in 3T3-L1 cells. Cells were incubated with differentiation cocktail (DMI) medium with Roflumilast (3 and 6 μM). (A) Cells were stained with Oil Red O on day 8; (B) Lipid accumulation was examined by measuring absorbance at 540 nm of Oil Red O (*, **, ***, P < 0.05, 0.01, 0.005 vs. vehicle group; ##, ###, P < 0.01, 0.005 vs. DMI group, n = 6).
