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. 2021 Jun 7;12:662451. doi: 10.3389/fendo.2021.662451

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Copyright © 2021 Xu, Zhang and Xiao

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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AMPKα mediates adipogenesis. (A) Cells were incubated with a differentiation cocktail (DMI) medium with Roflumilast (6 μM). Phosphorylated and total levels of AMPKα were measured, and the representative images of each protein were shown on the top panel, the quantification of p-AMPKα/AMPKα ratio was shown on the bottom panel; (B) Cells were incubated with a differentiation cocktail (DMI) medium with Roflumilast (6 μM) or the compound C (10 μM) for 8 days. mRNA of PPAR-γ and C/EBPα; (C) Lipid accumulation was examined by measuring absorbance at 540 nm of Oil Red O (**, P < 0.05 vs. vehicle group; ***, ^###, ^$$$, P < 0.005 vs. vehicle, DMI group, and DMI+ Roflumilast group, respectively, n = 4-5).