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. 2021 Jun 7;12:616451. doi: 10.3389/fimmu.2021.616451

Figure 1.

Figure 1

Several alternative MyD88 isoforms support NF-κB signaling. (A, B) Schematic representation of MYD88 isoforms on mRNA (A) and protein (B) level according to references in Table 1 . (B) Illustration of target epitopes of the different antibodies used in this study. (C–E) HEK293T cells were transfected with plasmids for different MYD88 splice isoforms and lysates analyzed for expression or pathway activation by immunoblot (C, n=3) or NF-κB dual luciferase assay (D, n=4), respectively. (E) as in D but using MyD88-deficient I3A cells (n=3). (F) Immunoblot of primary B cell lysates from two different donors, lysates of HEK293T transfected with untagged isoforms 1 to 5 (‘Isof. 1-5 ladder’) and MyD88-competent or deficient (KO) THP-1 reporter cells. In C-E one representative of ‘n’ technical replicates is shown as mean + SD from three repeats. ns, non-significant; * p<0.05 according to two-way ANOVA comparing to isoform 1 (D, E).