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. 2021 Jun 7;12:616451. doi: 10.3389/fimmu.2021.616451

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Copyright © 2021 Cardona Gloria, Bernhart, Fillinger, Wolz, Dickhöfer, Admard, Ossowski, Nahnsen, Siebert and Weber

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TLR stimulation induces isoform 3 only transiently in primary B cells. (A–B) RT-qPCR analysis of isoforms 1/2 to 5 in primary B cells (A, n=5-7) and hMoMacs (B, n=3) stimulated with LPS or CpG as indicated (n=3). (C) RT-qPCR analysis of isoforms 1/2 to 5 in primary B cells stimulated with CpG and IgM to induce proliferation (n=3). A-C represent combined data (mean+SD from ‘n’ biological replicates (each dot represents one replicate). ns, non-significant; * or # = p<0.05 according to Kruskal-Wallis test (A), ordinary one-way ANOVA (B) or two-way ANOVA (C).