Figure 2.
. Cytotoxic activity of TNFα, Tag7-Hsp70, IgG in the presence of inhibitory peptides. Inhibitory 17.1, 17.1А, 17.0 peptides were added at a concentration 10−9 M 1 h prior to incubation with the cytotoxic agent TNFα 10−10 M (A), Tag7-Hsp70 10−9 M (B), IgG 10−10 M (C). Anti-TNFR1 antibodies (1: 100) were added to the cells also 1 h prior to incubation with the cytotoxic agents (control). The cytotoxic activity was measured 3 and 24 h after incubation of cells with the cytotoxic agent using trypan blue staining. All data are presented as mean ± SEM for at least three independent replicates. (*p<0.05 t-test vs control).