Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jun 21;40:200. doi: 10.1186/s13046-021-01994-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 2 — Colon-specific knockout of IMP3 decreased AOM/DSS induced colon carcinogenesis. (A) Scheme of Villin-Cre-mediated recombination of IMP3 floxed allele. (B) PCR amplification of genomic DNA. Villin-Cre IMP3^−/− colon shows a 666 base pair band specific to recombination allele. (C) The quantitative real-time RT-PCR analysis of Villin-Cre IMP3^−/−colon tissues shows an approximately 90% decrease of IMP3 mRNA levels compared with the IMP3^fl/fl colon tissues. (D) Six- to 8-week-old IMP3^fl/fl (n = 9) and Villin-Cre IMP3^−/−mice (n = 9) were subjected to a single intraperitoneal injection of AOM (10 mg/kg/body weight) followed by 3 cycles of 1 week of administration of 2.5% DSS in the drinking water, each cycle separated by a 2-week period. Mice were killed at 12 weeks after AOM/DSS exposure. (E) HE staining of representative colon tissue image of Villin-Cre IMP3^−/− and IMP3^fl/fl mice. (F) Prevalence of low-grade adenomas, highgrade adenomas, and adenocarcinomas of Villin-Cre IMP3^−/− and IMP3^fl/fl mice. (G) Macroscopic finding of Villin-Cre IMP3^−/− and IMP3^fl/fl mouse colons. (H)Tumor areas, Villin-Cre IMP3^−/− mice showed a significant reduction in the total tumor areas compared with IMP3^fl/fl mice. (I) Tumor numbers, Villin-Cre IMP3^−/− mice showed a significant reduction in the total tumor numbers(left) and per mouse tumor number(right) compared with IMP3^fl/fl mice. (J) Villin-Cre IMP3^−/− mouse colons showed a decreased total tumor numbers or per mouse tumor numbers in which the diameter was larger than 3.0 mm, while no significant difference was found in tumor diameter was smaller than 3.0 mm