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. 2021 Jun 21;40:200. doi: 10.1186/s13046-021-01994-8

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — IMP3 promoted CRC cell proliferation, migration and invasion in vitro. (A) The baseline RNA level of IMP3 in eight CRC cell lines detected by RT-qPCR. (B) The baseline protein level of IMP3 in eight CRC cell lines detected by western blot. (C) Efficiencies of IMP3 knockdown was detected in indicated cells transfected with siRNAs by RT-qPCR (* p < 0.05). (D) Efficiencies of IMP3 overexpression was detected in indicated cells transfected with plasmids by RT-qPCR (* p < 0.05). (E) Efficiencies of IMP3 knockdown or overexpression was detected in indicated cells transfected with siRNAs or plasmids by western blot. (F) CCK8 assays showed that knockdown of IMP3 reduced cell viabilty in RKO and HCT116 cell line (* p < 0.05), and upregulation of IMP3 increased cell viability in LOVO and SW480 cell line (* p < 0.05). (G) Colony formation assays for indicated cells after transfection with siRNAs or plasmids (* p < 0.05). (H) Representative images (40×) of wound healing assays for indicated cells (* p < 0.05). (I) Representative images (200×) of transwell invasion assay for indicated cells (* p < 0.05)