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. 2021 Jun 21;7:10. doi: 10.1186/s40851-021-00175-x

Fig. 4.

Fig. 4

 Expression of the genes responsible for NaCl (A and B) and water absorption (C) in the anterior intestine (AI), middle intestine (MI) and posterior intestine (PI) in FW-acclimated (plain column) and SW-acclimated (filled column) eels as determined by real-time qPCR. The expression of the genes related to Cl secretion suggested in mammals and other marine teleosts is also shown in (D). The expression levels are corrected by those of elf1a and thus indicate the relative abundance values of the genes. The atp1, kcnj2 and kcnj16 are the genes for NKA, Kir2.1 and Kir5.1, respectively. The figures with ‘Reads per million’ on the ordinate were created from RNA-seq data (n = 5). The figures are depicted based on the data in Ando et al. [12], Wong et al. [270], and Takei et al. [228] and on unpublished data. The primers for real-time PCR, including those for the unpublished data, are listed in Supplementary Table 1. *p < 0.05, **p < 0.01, ***p < 0.001. For details, see 4.1.1 and 4.2.1. For abbreviation definitions, see the list