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. 2021 Jun 21;40:203. doi: 10.1186/s13046-021-01989-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Target genes of OICR-9429 are identified in bladder cancer. a. Volcano plot showing the differentially expressed genes in RNA sequencing in T24 and UM-UC-3 cells treated with DMSO or 140 μM OICR-9429 for 48 h. b. A heatmap representing both downregulated mRNA expression levels in T24 and UM-UC-3 cells treated with OICR-9429. c. The differentially expressed genes in the RNA sequencing were verified in T24 and UM-UC-3 cells by qRT-PCR. d. The expression of OICR-9429 target genes in T24 and UM-UC-3 cells was detected by Western blots. GAPDH and histone H3 were used as internal controls. e. ChIP-qPCR analysis of H3K4 tri-methylation and Pol-II status in the promoters of target genes after treatment with DMSO or 140 μM OICR-9429 for 48 h in T24 and UM-UC-3 cells. Values were normalized to the DMSO groups. *p < 0.05 and **p < 0.01