Fig. 4.

ZIP13 knockout inhibits the growth of ovarian cancer cells in vitro. a, c. Cell proliferation assay was performed using CCK-8. At 0, 24, 48, 72 and 96 h, CCK-8 solution was added to each well and OD450 values were measured by a microplate reader. All conditions were tested in six replicates. b, d. SKOV-3 and HO-8910 PM cells were plated in 6-well plates and cultured for 2 weeks. Then the colonies were fixed and stained. The colony number was counted under an optical microscope. Each experiment was independently conducted at least three times. *P < 0.05