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Blind assay for the clinical performance of the heat shock method for SARS-CoV-2 detection without RNA extraction for CDC (N gene) and Charite (E gene) RT-qPCR protocol (% values of true positives is the sensitivity)
| RT-PCR protocol | true positive samples | false negative samples | total positive samples |
|---|---|---|---|
| N gene | 123 (69.9%) | 53 (30.1%) | 176 |
| E gene | 69 (90.8%) | 7 (9.2%) | 76 |
