KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals | ||
| Dulbecco’s Modified Eagle’s Medium (DMEM) | GenDepot, Barker, TX | Cat# CM001-050 |
| Fetal Bovine Serum (FBS) | GenDepot | Cat# F0900-050 |
| Penicillin-Streptomycin (P/S) | Sigma-Aldrich, St. Louis, MO | Cat# P0781 |
| jetPRIME reagent | Polyplus, Berkeley, CA | Cat# 55-133 |
| Alexa 555-C2- maleimide | Invitrogen, Carlsbad, CA | Cat# A20346 |
| Alexa 647-C2-maleimide | Invitrogen, Carlsbad, CA | Cat# A20347 |
| Lauryl Maltose Neopentyl Glycol | Anatrace, Maumee, OH | Cat# NG310 |
| n-Dodecyl-β-D-maltoside | Chem-Impex Int’l Inc., Wood Dale, IL | Cat# 21950 |
| Cholesteryl Hydrogen Succinate | MP Biomedicals, Solon, OH | Cat# 101379 |
| Pierce Protease Inhibitor Mini Tablets | ThermoFisher Scientific, Waltham, MA | Cat# A32953 |
| Liquinox phosphate-free detergent | Fisher Scientific | Cat# NC9906065 |
| Vectabond | Vector Laboratories, Burlingame, CA | Cat# SP-1800 |
| Biotin-terminated Polyethylene glycol (PEG) | NOF Corp., Tokyo, Japan | Cat# BI-050TS |
| mPEG succinimidyl carbonate | Laysan Bio Inc., Arab, AL | Cat# MPEG-SC-5000 |
| Sodium bicarbonate | Sigma-Aldrich | Cat# S6014 |
| MS(PEG)4 Methyl-PEG-NHS-Ester Reagent | Thermo Scientific | Cat # 22341 |
| Streptavidin | Invitrogen | Cat# 434301 |
| Glucose | Sigma-Aldrich | Cat# G8270 |
| Pyranose oxidase | Sigma-Aldrich | Cat# P4234 |
| Catalase | Sigma-Aldrich | Cat# C1345 |
| Ascorbic acid | Sigma-Aldrich | Cat# A7506 |
| Methyl viologen | Fisher Scientific | Cat# AC227320010 |
| Experimental Models: Cell Lines | ||
| HEK 293T cells | ATCC, Manassas VA | Cat# 3216 |
| Recombinant DNA | ||
| pcDNA3.1 | Invitrogen | Cat# V790-20 |
| Software and Algorithms | ||
| Origin Software | Origin Lab | |
| Matlab | MathWorks | |