Figure 3. Effect of acute H₂O₂-induced oxidative stress on cell viability and neuroprotective evaluation of either α-scoliidine or β-scoliidine in PC12 cells. PC12 cells were seeded at 2 x 10⁴ cells/well in a 96-well plate for 24 h, and then submitted to different treatment. (A) Neuroprotective assay scheme where cells were treated with α-scoliidine or β-scoliidine (1 µM) for 22 h at 37°C and, after the medium were replaced containing peptide and H₂O₂ (0.6 mM) and incubated for more 2h. (B) Dose-response of H₂O₂for concentrations varying between 1-0.06 mM for 2 h. (C) Protective effects of α-scoliidine or β-scoliidine against oxidative stress-induced neurotoxicity. Values are expressed as mean ± standard deviation from three independent experiments in triplicate and analyzed by one-way ANOVA followed by Tukey’s post-test. *p <0.05 to differences among the control group.