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. Author manuscript; available in PMC: 2021 Jun 21.
Published in final edited form as: Methods Mol Biol. 2018;1680:205–215. doi: 10.1007/978-1-4939-7339-2_14

Fig. 2.

Fig. 2

Generation of Ago2-deficient 3T3 MEFs. (a) A scheme showing a strategy to delete Ago2 in 3T3 Ago2flox/flox MEFs by adenovirus-mediated cre recombinase (Adeno-Cre) expression. (b) A western blot analysis assessing expression levels Ago2 detected by an anti-Ago2 monoclonal antibody in MEFs treated with Adeno-GFP and Adeno-Cre. β-Tubulin is shown as a control