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. 2021 Feb 10;7(3):227–240. doi: 10.1159/000512750

Fig. 6.

Fig. 6

FGF21 enhanced the calcification effect of PTH on HAECs via calcium deposition and EndMT. a CCK-8 assay for HAEC viability in different conditions. b Alizarin Red staining on HAECs stimulated by FGF21 with or without PTH co-incubation. Bright red or orange staining indicates a positive result for calcium deposition. c Expression of CD31 mRNA in HAECs stimulated by FGF21 with or without PTH co-incubation. d Expression of RUNX2 mRNA in HAECs stimulated by FGF21 with or without PTH co-incubation. e Expression of FSP1 mRNA in HAECs stimulated by FGF21 with or without PTH co-incubation. *p < 0.05 and **p < 0.01. EndMT, endothelial-to-mesenchymal transition; FGF21, fibroblast growth factor 21; PTH, parathyroid hormone; HAECs, human aortic endothelial cells; CCK-8, Cell Counting Kit-8.