Figure 5.

AKT knockdown reverses the anti- apoptotic effects and activation of the AKT/mTOR/NF-κB pathway induced by CD155 overexpression. CaSki and HeLa cells overexpressing CD155 were transfected with AKT-siRNA (A) Flow cytometry was used to detect apoptosis after staining with APC Annexin-V and 7AAD. The apoptotic ratio in CaSki and HeLa cells was quantitatively analyzed with FlowJo v10. (B) The expression of CD155, p-AKT, p-mTOR, Beclin-1, LC3BI, LCBII, p-IKKγ, p-IKKα/β, CL-caspase9, and Ki67 was measured by western blot. β-actin was used as a control. Quantitation was performed using Image J, and the data are the mean ± SEM of at least three independent experiments. *P < 0.05, **P < 0.01, and ***P < 0.001.