| 10% Sugar solution |
|
|
|
| 10ml serological pipet |
Falcon |
357551 |
|
| 15 ml conical tube |
Falcon |
352096 |
|
| 1ml serological pipet |
Falcon |
357521 |
|
| 25 ml serological pipet |
Falcon |
357535 |
|
| 37°C Incubator |
|
|
|
| 50 ml conical tube |
Falcon |
352070 |
|
| 5ml serological pipet |
Falcon |
357543 |
|
| 6 well tissue culture plates |
Falcon |
353046 |
|
| 70% Ethanol |
|
|
|
| 9″ glass pipet |
Fisherbrand |
13-678-6B |
|
| Anopheles Mosquitoes |
JHMRI, Insectary core |
|
We use A. stephensi or A. gambiae (keele) |
| cell counter |
|
|
|
| Circulating water bath |
|
|
|
| fine tip forceps |
Fisherbrand |
12-000-122 |
|
| Geimsa stain |
Sigma |
GS1L |
|
| Glass desiccator |
|
|
|
| Glass membrane feeder |
Chemglass Life Sciences |
CG183570 |
|
| Glass slides |
Fisherbrand |
12-552-3 |
|
| HBSS |
Sigma |
H6648 |
|
| Human Blood O+ |
JHU |
|
Wash RBCs three times with RPMI and refrigerate at 50% heamatocrit |
| Human Serum O+ |
Interstate blood bank |
|
Pool at-least 6 units of serum from different donors and freeze down aliquots at −20°C. |
| Hypoxanthine |
Sigma |
H9337 |
Make 500x stock in 1M NaOH |
| Mercurochrome |
Sigma |
M7011 |
Prepare 1% stock solution in PBS that can be diluted to 0.1% when needed |
| Micro Pipette |
|
|
|
| Microscope |
Olympus |
|
Any microscope with 10x, 40x and 100x objective will work. |
| Mosquito cups |
Neptune cups |
|
|
| N-acetylglucosamine |
Sigma |
A3286 |
Optional and needed only when pure gametocytes are required. |
| Netting |
|
|
Make sure it can contain mosquitoes and allow blood feeding |
| Parafilm |
|
|
|
| PBS |
|
|
|
| Petri dish |
Thermo Scientific |
249964 |
|
| Pipet tips |
|
|
|
| Pipetman |
|
|
|
|
Plasmodium falciparum NF54 |
BEI Resources |
MRA-1000 |
Freeze down large numbers of early passage culture to make sure you have a constant supply |
| RPMI 1640 |
Corning |
CV-041-CV |
Media contains glutamine and HEPES |
| Slide warmer |
|
|
|
| Sodium bicarbonate |
Sigma |
S6297 |
Optional for media, add only when using malaria gas mix during culture incubation |
| water bath |
|
|
|
| Xanthurenic Acid |
Sigma |
D120804 |
For flagellation media |
