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qRT-PCR for relative expression of hepatic BAX (a), TGF-β (b), and protein expression of P-glycoprotein by ELISA (c), respectively. *,#Significantly different from control, MTX, and MTX-gossypin-treated groups, respectively, at P < 0.05. TGF-β=transforming growth factor-beta, MTX = methotrexate, QRT-PCR = Quantitative real-time polymerase chain reaction
