Figure 4.

Anti-miR-27a-3p treatment reduced collagen in SSc fibroblasts. (a) SSc dermal fibroblasts were cultured and transfected with antagomiR27a-3p or scramble (80 nM) controls and after 48 h the media was removed and collagen quantified by Sircol assay. Data is the percentage of scramble control after 48 h transfection and is the mean and SD *Significant difference versus scramble control, (P = <0.0001; students t test n = 4). (b) Representative western blot of collagen1 after scramble control microRNA or antagomiR27a-3p after 48 h. β-actin is the loading control. Scr = Scramble, Anti-miR = AntagomiR27a-3p. (c) SSc dermal fibroblasts were transfected with antagomiR27a-3p or scramble controls and after 48 h MTT assay was performed. No significant difference between groups was observed P = >0.05; students t test, n = 4)