Figure 2.

Global epigenetic alterations in Obese compared to Lean MSCs demonstrated by: a) dot-blotting of DNA hydroxymethylation measuring genomic 5-hydroxymethyl-cytosine (5hmC), b) and Western blotting of histone-3 protein measuring genomic tri-methylation on lysine-4 (H3K4me3), 9 (H3K9me3), and 27 (H3K27me3) residues. Genomic 5hmC and H3K4me3 were reduced in Obese compared to Lean MSCs, but there were no different in Global H3K9me3 and H3K27me3 between Lean and Obese MSCs. *p ≤ 0.05 vs. Lean MSC