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. 2021 Jun 7;11:688007. doi: 10.3389/fcimb.2021.688007

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Copyright © 2021 Zhang, Guo, Cui, Chen, Zhang, Wang, Li, Fu, Wang, Guo, Liu and Dong

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The agglutination activities of the WT and MA-P5 viruses in three types of chicken red blood cells (cRBCs) and sheep red blood cells (sRBCs) were determined by HA titer. cRBCs, cRBCs with α-2,3-linked sialic acid receptors and α-2,6-linked sialic acid receptors; α-2,3 cRBCs, cRBCs treated with α-2,3-sialidase (with only α-2,6-linked sialic acid receptors); α-2,6 sRBCs, sRBCs (with only α-2,3-linked sialic acid receptors); and desialylated (desial) cRBCs, cRBCs treated with VCNA (no receptors). The values are shown as the means and standard deviations of three independent experiments. *P < 0.05 (MA-P5 vs WT).