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Representative images of immunohistochemical staining (H&E staining) in the middle ear cavity and tympanic membrane of guinea pigs 28 days after USMB treatment. Positive controls treated with one intratympanic injection of 200 μL of LPS (200 μg/mL) presented with acute otitis media with effusion and were sacrificed on post-treatment day 7. ITS, intratympanic saline injection without MBs; E, epithelium; LP, lamina propria.
