Figure 4.

The inhibitory effect of LF-1 and LF-2 on the formation of S. mutans biofilm (A, B), as well as the eradication of 1-d preformed S. mutans biofilm by LF-1 and LF-2 (C, D) are illustrated by the absorbance at 595 nm and CFU number, respectively. Columns labelled with different superscript letters denote significant statistical differences among treatments (One-way ANOVA, p < 0.05). For the biofilm inhibition assay (A, B), representative images of stained biofilms treated with peptides at concentrations ranging from 64 to 0 μM are presented above the columns