Figure 1.

(A) Schematic illustration of the concept that aptameric enzyme subunit enhances myoglobin-derived peroxidase reaction. The aptameric enzyme subunit binds to myoglobin directly and enhances the performance of peroxidase activity, which catalyzes H₂O₂ and luminol. (B) Selection of aptameric enzyme subunit from the myoglobin-binding aptamers obtained by the systematic evolution of ligands by exponential enrichment. The aptameric enzyme subunit was selected by the investigation of aptamer-captured myoglobin. After the removal of free-myoglobin, chemiluminescent signal derived from the catalytic reaction of myoglobin trapped by the aptamer was analyzed with luminol. The mean ± SD is shown in the graph (N = 3). (C) Sequence of N24-07 except for the primer-binding site and circular dichroism spectrum of N24-07. Three consecutive guanines are underlined.