Table 1.
NMR refinement statistics
| U1-A RRM2 linker | |
|---|---|
| NMR distances and dihedral constraints | |
| Distance constraints | |
| Total NOE | 1840 |
| Intra-residue | 387 |
| Inter-residue | 1453 |
| Sequential (|i – j| = 1) | 444 |
| Medium-range (1<|i – j| < 4) | 308 |
| Long-range (|i – j| > 5) | 671 |
| Hydrogen bonds | 30 |
| Total dihedral angle restraints | |
| ϕ | 72 |
| ψ | 72 |
| Structure statistics | |
| Violations (mean ± s.d.) | |
| Distance constraints (Å) > 0.4 Å | 0.30 ± 0.50 |
| Dihedral angle constraints (°) > 5° | 5.00 ± 1.40 |
| Max. dihedral angle violation (°) | 7.49 ± 2.67 |
| Max. distance constraint violation (Å) | 0.37 ± 0.13 |
| Deviations from idealized geometry | |
| Bond lengths (Å) | 0.0035 ± 0.0012 |
| Bond angles (°) | 1.443 ± 0.481 |
| Ramachandran plot statistics | |
| Residue in most favored regions (%) | 84.4 |
| Residue in additionally allowed regions (%) | 15.6 |
| Residue in generously allowed regions (%) | 0.0 |
| Residue in disallowed regions (%) | 0.0 |
| Average pairwise r.m.s. deviation (Å) | |
| Heavy | 0.12 ± 0.05a |
| Backbone | 0.50 ± 0.06a |
aPairwise RMSD calculated among the 20 NMR structures using residue range 208–240, 244–282.