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. 2021 Jun 9;49(11):6456–6473. doi: 10.1093/nar/gkab484

Figure 4.

Figure 4.

RP-CONA detects the specific interaction of pri-miR-7–1-CTL/HuR. (A) A diagram illustrating that mCherry-HuR interacts with FITC-pri-miR-7–1-CTL but not FITC-pri-miR-7–1/30a-TL. (B) mCherry-HuR pull-down by beads coupled with 40 pmol of FITC-pri-miR-7–1-CTL or FITC-pri-miR-7–1/30a-TL. (C) Pri-miR-7–1-CTL binds mCherry-HuR but not mCherry. FITC-pri-miR-7–1-CTL beads were incubated with cell extracts containing 300 nM mCherry-HuR or mCherry. (D and E) mCherry/FITC signals were reduced by anti-HuR antibody. Cell extracts containing 300 nM mCherry-HuR were pre-incubated with 2 μl (+) or 4 μl (++) of anti-Lin28a antibody (control) or anti-HuR antibody prior pulldown by FITC-pri-miR-7–1-CTL-beads. Mean mCherry/FITC ring intensities and SD between triplicates are shown. Statistically significant differences compared to anti-Lin28a antibody treated samples were interpreted by SPSS independent sample t-test, **P< 0.01, ***P<0.001.