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. 2021 Jun 9;49(11):6587–6595. doi: 10.1093/nar/gkab487

Figure 3.

Figure 3.

Binding of AcrIIA14 to the HNH domain induces SauCas9 allostery. (A) Structural comparison of SauCas9–sgRNA–dsDNA complexes in the bound and unbound states of AcrIIA14ct. The HNH domains in SauCas9–sgRNA–dsDNA and SauCas9-sgRNA-AcrIIA14ct-dsDNA are colored blue and cyan, respectively. The L1 linkers in SauCas9–sgRNA–dsDNA and SauCas9–sgRNA–AcrIIA14ct–dsDNA are colored orange and yellow, respectively. (B) Local structure of AcrIIA14 induces SauCas9 allostery. The black boxes represent two new intramolecular connections. Above, shows interaction of SauCas9 HNH domain with the target DNA strand. Below, shows interaction of SauCas9 L1 linker with sgRNA. (C) Detailed interaction between the SauCas9 HNH domain with the target DNA strand. (D) Detailed interaction between the L1 linker with sgRNA.