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. 2021 Jun 18;30:09636897211024183. doi: 10.1177/09636897211024183

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© The Author(s) 2021

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 3. — The administration of hAMSCs suppresses pro-inflammatory immune cells around the peri-infarct area. (a) Tissue section stained with 4′,6-diamino-2-phenylindole (DAPI) showing regions of analysis in the peri-infarct area ((a) ischemic core). Three random regions, indicated by solid boxes (200× magnification), per coronal section and two coronal sections per brain were analyzed to count iNOS+/CD11bc+ cells [M-high: n = 3; M-vehi: n = 3]. Scale bar: 200 µm. (b) Representative images of the immunofluorescent staining of brain sections in the M-high and M-vehi mice. iNOS (green) was colocalized (yellow) with CD11b/c (red). Nuclei were counterstained using DAPI (blue). Scale bar: 50 µm. 200× magnification. (c) Cell count of iNOS positive, CD11b/c positive, and iNOS/CD11bc colocalized cells. Scale bar: 50 µm. **P < .01, ***P < .001, unpaired t-test.