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. 2020 Jun 3;27(1):805–815. doi: 10.1080/10717544.2020.1770371

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© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Effect of CaCl₂ on the association of pGFP-Cy5 polyplexes and Jurkat cells in a 48 h culture analyzed by flow cytometer. “CO” is a cell only control; “0” is a polyplex only control; “10 Ca” and “25 Ca” are polyplex formulations with 10- and 25 mM CaCl₂, respectively; “10 Mg” and “25 Mg” are polyplex formulations with 10- and 25 mM MgCl₂, respectively; and ”LF” is a lipoplex only condition. (a) Confocal laser scanning microscope maximum intensity projection image of Jurkat cells associated with polyplexes; Scale bar in every image is 2 μm; Nucleus is stained with DAPI (blue) and pGFP is labeled with Cy5 (red). (b, c) All comparisons are made with “0” group of respective time point; Results are presented as mean ± SD (n = 3; 1-way ANOVA with Dunnett’s multiple comparisons, *** p = .0005 and 0.0009, **** p <.0001).