Figure 4.

Extended splice correction effect of PPMO plus OEC in murine lung. EGFP654 mice were retained as controls or received PPMO654 at 5 mg/kg IV on two successive days and thereafter one cohort received OEC (UNC7938) IV at 15 mg/kg once (n = 3–4 per group). After 2, 7 or 22 days mice were euthanized, lung tissues recovered and analyzed by RT-PCR as described in Methods. (A) PCR Gel analysis at 48 h (top) and 7 and 22 days. (B) Quantitation of splice correction as described in Methods; shown vehicle and PPMO at 48 h; *P < 0.01 versus no OEC.