Figure 4.

Characterization of homo- and heterochiral toehold interactions. (A) The model system used to monitor toehold association based on fluorescence quenching. (B) Fluorescence melting curves for the homo- and heterochiral toehold duplexes (C_t = 2 μM). Fluorescence values were corrected (F_corr) for background fluorescence and temperature-dependent effects as defined in eq 4 (see Materials and Methods). The T_m for each toehold configuration was averaged over three melting experiments. (C) Thermodynamic parameters for homo- and heterochiral toehold association.