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. 2021 Mar 8;49(11):e64. doi: 10.1093/nar/gkab144

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 1. — Schematic depiction of aniFOUND. An asynchronous population of fibroblasts is synchronized to G0/G1 phase by serum starvation and contact inhibition. DNA photolesions are formed by UVC-irradiation and left to be repaired in the presence of EdU. HU is added in this step to eliminate the replication of any escaper cells. Since no replication occurs, UDS is the only source of DNA synthesis. Biotin molecules are conjugated to EdU, the chromatin or the extracted DNA is sheared and the biotin-labelled fragments are isolated with streptavidin beads. The captured material can be used for several further analyses.